Journal: Diabetology & Metabolic Syndrome
Article Title: Identification of the LncRNA PRNCR1/miR-642a-5p/SSTR3 CeRNA network and its diagnostic value in type 2 diabetes mellitus
doi: 10.1186/s13098-025-02061-4
Figure Lengend Snippet: PRNCR1 modulates SSTR3 via sponge adsorption of hsa-miR-642a-5p. ( A ) Results of the cytoplasmic and nuclear PRNCR1 isolation experiment. GADPH is a cytoplasmic location marker, and U6 is a nuclear location marker. ( B ) The binding sites of PRNCR1 or SSTR3 and hsa-miR-642a-5p. ( C ) Verification of the binding of PRNCR1 with hsa-miR-642a-5p via a luciferase assay. ( D ) Verification of the binding of SSTR3 with hsa-miR-642a-5p via a luciferase assay. ( E and F ) Anti-ago2 RIP analysis was performed, followed by qRT-PCR to detect PRNCR1 and hsa-miR-642a-5p. * indicates P < 0.05
Article Snippet: The cells were lysed with RIPA lysate and incubated with magnetic beads conjugated with human antibody against Ago2 (Proteintech) or normal mouse IgG (Millipore).
Techniques: Adsorption, Isolation, Marker, Binding Assay, Luciferase, Quantitative RT-PCR